Thermo Scientific BRAHMS PCT LIA

Thermo Scientific BRAHMS PCT LIA

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Immunoluminometric assay (ILMA) for the quantitative determination of Procalcitonin (PCT) in human serum and plasma

 

Assay characteristics

  • One-step immunoluminescence assay using coated tube systemwith two monoclonal antibodies (sandwich principle)
  • Sample matrix: Serum or plasma
  • Sample volume: 20 μL
  • Incubation time: 1 hour at room temperature (18-25 °C)
  • Measuring range: 0.1-500 μg/L
  • Functional assay sensitivity: 0.3 μg/L
  • High-dose hook: No high-dose hook effect detectable up to 900 μg/L

 

Assay procedure

  • Pipette 20 μL sample plus 250 μL tracer into the coated tube
  • Incubate for 1 hour at room temperature (18-25 °C)
  • Remove excess tracer by thorough washing and subsequently remove the remaining liquid (put tube upside down on filter paper for 5-10 min)
  • Measure luminescence for 1 sec in a luminometer
  • Calculate results from the standard curve

 

Reproducibility

The inter-assay precision in the clinically relevant range is between 6 and 10% CV. The functional assay sensitivity, defined as the smallest value with an inter-assay precision of 20% CV, is 0.3 μg/L.

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