Leveraging the historically stable fluidic design of the original MoFlo and the electronic processing of the MoFlo XDP, the MoFlo Astrios expands sorting capabilities with a full palette of laser options. Seven pinholes spatially separate each laser, providing the flexibility and expandability known to MoFlo sorting. The MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates daily set up with its operation designed around core laboratory workflow. Auto Start Up enables the user to pre-define when the lasers and fluidics will start up, saving valuable time each morning. Creating flat-top beam profiles, a multi-fiber Beam Shaping Optic (BSO) shortens alignment time and improves optic stability. Two stages control the alignment for all 7 lasers, simplifying the sorter preparation.