The AB Library Builder System simplifies sequencing of DNA fragments and RNA whole transcriptome. The semi-automated system simplifies sequencing and carries out the most labor intensive processes. This considerably increases throughput and reduces the hands-on time by 50% for library preparations such as whole transcriptome, exome and targeted resequencing applications. It can prepare up to 13 libraries per run and up to 26 per day. The integrated, cost-effective system offers a complete solution for library preparation for the 5500 Series Genetic Analysis Systems and the SOLiD 4 System. Other features include the predefined software protocol and plug-and-play cartridge format reagents.