The P3D chambers have been designed to hold porous cylindrical scaffolds subjected to perfusion culture conditions. They have been conceived as disposable chambers and are supplied in sterile ready-to-use conditions aiming to facilitate the experimental set-up building process. The chambers are composed of two parts which hold the scaffold and fix it in the right position so the flow is homogeneously distributed through its whole volume. Two sizes are available to adapt to scaffolds of different diameters and lengths.