These columns are designed to be safe to use and easy to work with. This is made with a weir structure to keep the stationary setup intact and to use the right particles as necessary. The weirs are easier to build and fabricate as the dead volume is at rates of close to zero. These columns are better to use than ones that are made with fused silica tubes. These are made with easy handles and can be linked to units without creating dead volumes. It has a plug and play setup and can go from one workflow to the next. The setup is reproducible and can run with just about every review parameter possible.