FC50A / B use totally closed membrane filter sections repeatedly, can be in concert with various HTY intelligent sterility test pump. The test solution can be filtered through directional motility pressure effect of HTY intelligent sterility test pump. Micro-organisms of test solution can be trapped on the membrane. After filtration, take the repeated used closed membrane filter apart, remove the membrane to culture medium. Then detect and count, by this, the need to reduce costs of microbial detection can be met.