Shimadzu has released the e-Series comprehensive 2D gas and liquid chromatographs. The new systems maximize the uppermost features of chromatography, separation performance, and detection, and achieve high-resolution analysis, even for structurally similar compounds. The e-Series consists of the Nexera-e LCxLC system and the Ultra-e GCxGC system. Dedicated ChromSquare software is used for data analysis. The e-Series are the commercialized products resulting from our collaboration with professor Luigi Mondello at the University of Messina in Italy, who is renowned for his advanced research in the field of comprehensive 2D chromatography. Combining two different separation systems, the e-Series enables the separation of target substances in complex samples containing a large number of components. These systems are ideal for a variety of fields, such as foods, flavors and fragrances, environmental, petroleum, and chemical, where there is an increasing need for expeditious and comprehensive analysis of small differences in complex samples. Shimadzu is offering the column switching two-dimensional LC system "Co-Sense system", the column switching two-dimensional GC system "MD-GC system" and others. These systems enable the detailed quantitative analysis of target compounds specified with the comprehensive screening with e-Series. With these multidimensional systems, from the exploratory research to the precise analysis, Shimadzu offers highest separation technologies appropriate for the customer's workflow.