sphingotest^® bio-ADM

Immunoluminometric Assay (ILMA) for the determination of bioactive Adrenomedullin (bio-ADM) in human EDTA plasma

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Bioactive Adrenomedullin (bio-ADM)

bio-ADM is the bioactive form of Adrenomedullin, a soluble 52 amino acid peptide hormone that is mainly released by vascular smooth muscle and endothelial cells. bio-ADM is an important regulator of vasodilation, blood pressure and organ perfusion [1] [2].

Prediction and Early Diagnosis of Shock & Guidance of Vasopressor Therapy

Elevated bio-ADM plasma levels predict numerous adverse endpoints for patients in acute care settings such as sepsis [3], acute myocardial infarction [4], and acute heart failure [5], as well as vasopressor demand in patients with septic shock [3], independently of other risk factors.

Testing principle

sphingotest® bio-ADM is an immunoluminometric assay (ILMA) for the determination of bio-ADM in human EDTA plasma. It represents the only available in vitro diagnostic (IVD) assay for the measurement of bioactive Adrenomedullin. One of the two sandwich antibodies is luminescent-labelled (tracer antibody) and highly specific for the amidated C-terminus of bio-ADM. The other antibody is immobilized to the wells of the microtiter plate (capture antibody) and specific for an internal epitope that is distant to the degradation-prone N-terminus of bio-ADM, resulting in increased analyte stability.

References

[1] Hinson et al (2000) Endocr Rev. 21(2):138-67.

[2] Bełtowski and Jamroz (2004) Pol J Pharmacol. 56(1):5-27.

[3] Marino et al. (2014) Crit Care. 18(1):R34.

[4] Yuyun et al. (2016) J Cardiovasc Med. [ahead of print]

[5] Self et al. (2016) Am J Emerg Med. 34(2):257-62.