MicroCal PEAQ-ITC is designed for ease-of-use and exceptional sensitivity. The system directly measures heat released or absorbed during biochemical binding events, from which it calculates binding affinity (KD), stoichiometry (n), enthalpy (?H), and entropy (?S). With minimal sample preparation and system optimization data is generated quickly and easily. The wide affinity range enables analysis of weak to high affinity binders, with excellent reproducibility. MicroCal ITC is an essential tool for any research laboratory studying biomolecular interactions where high sensitivity and fast results are paramount. User-friendly guided workflows with embedded help videos give any level of user the ability to generate high quality data. High signal to noise gives more confidence in accessing data quality and relevance of generated affinity and thermodynamic parameters. Automated washing with detergent of the sample cell and titration syringe assists in producing high quality reproducible data. All binding parameters (affinity, stoichiometry, enthalpy and entropy) in a single experiment. Quick to first results with no assay development, no labeling, no immobilization and no molecular weight limitations. Sensitivity to investigate any biomolecular interaction using as little as 10µg of protein. Directly measures millimolar to nanomolar affinities (KDS) (10-2 to 10-9 M). Measures nanomolar to picomolar disassociation constants using competitive binding techniques (10-9 to 10-12M). Nonreactive Hastelloy for chemical resistance and compatibility with biological samples. Compatible with non-aqueous solvents. Upgradable to fully automated MicroCal PEAQ-ITC Automated.
- Application: : isothermal titration