The development and successful commercialization of protein-based biopharmaceuticals and diagnostic reagents frequently depends on the ability to adequately characterize these complex biomolecules. Waters ACQUITY® XBridge™ BEH300 C4 reversed-phase columns and associated methods can help improve your protein separation and characterization challenges. XBridge BEH300 C4 Reversed-Phase HPLC Columns Wide pore, Ethyl Bridged Hybrid (BEH) based 3.5 um particles with C4 ligand well suited for HPLC-based protein and polypeptide characterization.