Polymerase Chain Reaction is fundamental to relatively all applications requiring high copy number of starting material. This product is utilized in all laboratories working with DNA and RNA. Polymerase Chain Reaction is a process where millions of copies of DNA are amplified from a single copy, or low copy number template. In extension to good laboratory practice. After preparation of the final reaction mix, tubes should be transferred to the amplification area, repeatedly through a pass box or in a closed container. Ideal PCR laboratory should consist of three areas, each isolated from the other. Because of the high copy number that is generated during PCR, it is exactly needed to prevent contamination of the PCR reaction. Reagents should be equipped in the reagent preparation area and transferred to the sample preparation area, through a pass box, or inside closed containers.