Glove Leak Tester is the glove in an isolator constituting the weakest link in containment barrier. It enables "in-situ" glove testing without interrupting the isolator's integrity by testing the glove outside. With the help of oxygen rising monitoring technique, this system gives the most precise glove testing equipment. Its key features are: Simple and fast to operate, where the test-time is around 6 minutes. The equipment can detect a perforation which is not visible with bare-eyes. On the testing chamber the glove is sealed and docked at the hand cuff level. After cleansing it, the volume gets reduced to negative reference pressure and oxygen level is analyzed. The Glove Leak Tester is movable and can be transferred from glove to glove. There are special extensions which enable to reach to high located gloves.